![]() Barcodes can be introduced in at least three ways. In general, the workflow of studies using barcoding technology consists of several main steps including 1) tag samples of interest with unique barcodes, 2) multiplex samples, 3) proceed barcoded samples by sequencers or other high-throughput techniques, and 4) demultiplex readouts and assign each sample to the corresponding barcode. ![]() Sequence reads that contain different barcodes illustrate different origins of molecules, whereas sequence reads with the same barcodes are the result of PCR duplication from the same original molecule ( Peng et al., 2015). The concept of the barcoding strategy is that individual original DNA or RNA fragments within the same pool of samples are tagged with a unique sequence of a molecular tag ( Peng et al., 2015). ![]() Barcodes are usually in the string form of random nucleotides, partially degenerate nucleotides, or defined nucleotides. In the past, barcodes have been given various names, such as unique molecular identifier (UMI) ( Kivioja et al., 2012), primer ID ( Jabara et al., 2011), and duplex barcodes. Finally, and importantly, two practical ideas about how barcodes can be further applied in studying SARS-CoV-2 to accelerate our understanding of this virus are proposed.ġ Introduction: the general principle of the barcoding strategyīarcoding strategy has first proposed to solve the problems of PCR duplications and to improve the accuracy of next-generation sequencing quantification ( Casbon et al., 2011 Kinde et al., 2011). In the meantime, I provide the viewpoint to improve the current COVID-19 diagnostic strategy with potential solutions. In this Mini-Review, I first explain the general principle of the barcoding strategy and then put forward recent studies using this technology to accomplish COVID-19 diagnostics and basic research. Essentially, this technology can also be used together with different biotechnologies, allowing for investigation with resolution of single molecules. To date, barcoding technology followed by high-throughput sequencing has been made for molecular diagnostics for SARS-CoV-2 infections because it can synchronously analyze up to tens of thousands of clinical samples within a short diagnostic time. Barcodes are molecular tags, that allow proceeding an array of samples at once. Here, the latest 26 articles using barcoding technology for COVID-19 diagnostics and biologically-relevant studies are reviewed. The critical question would be how to scale up testing capacity and perform a diagnostic test in a high-throughput manner with robust results and affordable costs. ![]() The outbreak of SARS-CoV-2 has made us more alert to the importance of viral diagnostics at a population level to rapidly control the spread of the disease. Epigenetics of Infectious Diseases Research Group, Center for Population Diagnostics, Łukasiewicz–PORT Polish Center for Technology Development, Wrocław, Poland. ![]()
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